Chromosome Missegregation in Single Human Oocytes Is Related to the Age and Gene Expression Profile.

ABSTRACT: The growing trend for women to postpone childbearing has resulted in a dramatic increase in the incidence of aneuploid pregnancies. Despite the importance to human reproductive health, the events precipitating female age-related meiotic errors are poorly understood. To gain new insight into the molecular basis of age-related chromosome missegregation in human oocytes, we combined the transcriptome profiles of twenty single oocytes (derived from females divided into two groups according to age <35 and ≥35 years) with their chromosome status obtained by array comparative genomic hybridization (aCGH). Furthermore, we compared the transcription profile of the single oocyte with the surrounding cumulus cells (CCs). RNA-seq data showed differences in gene expression between young and old oocytes

SUBMITTER: Barone S 

PROVIDER: S-EPMC7139522 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature