Apoptotic-Induced Effects of Acacia Catechu Willd. Extract in Human Colon Cancer Cells.

ABSTRACT: The research for innovative treatments against colon adenocarcinomas is still a great challenge. Acacia catechu Willd. heartwood extract (AC) has health-promoting qualities, especially at the gastrointestinal level. This study characterized AC for its catechins content and investigated the apoptosis-enhancing effect in human colorectal adenocarcinoma HT-29 cells, along with its ability to spare healthy tissue. MTT assay was used to describe the time course, concentration dependence and reversibility of AC-mediated cytotoxicity. Cell cycle analysis and AV-PI and DAPI-staining were performed to evaluate apoptosis, together with ROS formation, mitochondrial membrane potential (MMP) changes and caspase activities. Rat ileum and colon rings were tested for their viability and functionality to e

SUBMITTER: Chiaino E 

PROVIDER: S-EPMC7139529 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature