Effects of High-Dose Ionizing Radiation in Human Gene Expression: A Meta-Analysis.

ABSTRACT: The use of high-dose Ionizing Radiation (IR) is currently one of the most common modalities in treatment of many types of cancer. The objective of this work was to investigate the effects of high-dose ionizing radiation on healthy human tissue, utilizing quantitative analysis of gene expression. To this end, publicly available transcriptomics datasets from human samples irradiated with a high dose of radiation and non-irradiated (control) ones were selected, and gene expression was determined using RNA-Seq data analysis. Raw data from these studies were subjected to quality control and trimming. Mapping of RNA-Seq reads was performed by the partial selective alignment method, and differential gene expression analysis was conducted. Subsequently, a meta-analysis was performed to select diff

SUBMITTER: Kanakoglou DS 

PROVIDER: S-EPMC7139561 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature