TRNA Queuosine Modification Enzyme Modulates the Growth and Microbiome Recruitment to Breast Tumors.

ABSTRACT:

Background

Transfer RNA (tRNA) queuosine (Q)-modifications occur specifically in 4 cellular tRNAs at the wobble anticodon position. tRNA Q-modification in human cells depends on the gut microbiome because the microbiome product queuine is required for its installation by the enzyme Q tRNA ribosyltransferase catalytic subunit 1 (QTRT1) encoded in the human genome. Queuine is a micronutrient from diet and microbiome. Although tRNA Q-modification has been studied for a long time regarding its properties in decoding and tRNA fragment generation, how QTRT1 affects tumorigenesis and the microbiome is still poorly understood.

Results

We generated single clones of QTRT1-knockout breast cancer MCF7 cells using Double Nickase Plasmid. We also established a QTRT1-knockdown breast MDA-M

SUBMITTER: Zhang J 

PROVIDER: S-EPMC7139606 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature