Proteomics Analysis Reveals that Warburg Effect along with Modification in Lipid Metabolism Improves In Vitro Embryo Development under Low Oxygen.

ABSTRACT: The molecular mechanism regulating embryo development under reduced oxygen tension remains elusive. This study aimed to identify the molecular mechanism impacting embryo development under low oxygen conditions. Buffalo embryos were cultured under 5% or 20% oxygen and were evaluated according to their morphological parameters related to embryo development. The protein profiles of these embryos were compared using iTRAQ-based quantitative proteomics. Physiological O₂ (5%) significantly promoted blastocyst yield, hatching rate, embryo quality and cell count as compared to atmospheric O₂ (20%). The embryos in the 5% O₂ group had an improved hatching rate of cryopreserved blastocysts post-warming (p < 0.05). Comparative proteome profiles of hatched blasto

SUBMITTER: Shahzad Q 

PROVIDER: S-EPMC7139666 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature