Complex Size and Surface Charge Determine Nucleic Acid Transfer by Fusogenic Liposomes.

ABSTRACT: Highly efficient, biocompatible, and fast nucleic acid delivery methods are essential for biomedical applications and research. At present, two main strategies are used to this end. In non-viral transfection liposome- or polymer-based formulations are used to transfer cargo into cells via endocytosis, whereas viral carriers enable direct nucleic acid delivery into the cell cytoplasm. Here, we introduce a new generation of liposomes for nucleic acid delivery, which immediately fuse with the cellular plasma membrane upon contact to transfer the functional nucleic acid directly into the cell cytoplasm. For maximum fusion efficiency combined with high cargo transfer, nucleic acids had to be complexed and partially neutralized before incorporation into fusogenic liposomes. Among the various neu

SUBMITTER: Hoffmann M 

PROVIDER: S-EPMC7139958 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature