Omentin-1 Ameliorated Free Fatty Acid-Induced Impairment in Proliferation, Migration, and Inflammatory States of HUVECs.

ABSTRACT:

Objectives

Endothelial cell injury is a critical pathological change during the development of atherosclerosis. Here, we explored the effect of omentin-1 on free fatty acid- (FFA-) induced endothelial cell injury.

Methods

An FFA-induced endothelial cell injury model was established to investigate the role of omentin-1 in this process. Cell proliferation was analyzed with the Cell Counting Kit assay and flow cytometry. Scratch and transwell assays were used to evaluate cell migration. Factors secreted by endothelial cells after injury were detected by western blotting, reverse-transcription quantitative polymerase chain reaction, and cellular fluorescence assay.

Results

Omentin-1 rescued the FFA-induced impaired proliferation and migration capabilities of human umbilic

SUBMITTER: Chen Y 

PROVIDER: S-EPMC7140148 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature