The whole blood phagocytosis assay: a clinically relevant test of neutrophil function and dysfunction in community-acquired pneumonia.

ABSTRACT:

Objective

To refine and validate a neutrophil function assay with clinical relevance for patients with community-acquired pneumonia (CAP).

Design

Two phase cross-sectional study to standardise and refine the assay in blood from healthy volunteers and test neutrophil phagocytic function in hospital patients with CAP.

Participants

Phase one: Healthy adult volunteers (n = 30). Phase two: Critical care patients with severe CAP (n = 16), ward-level patients with moderate CAP (n = 15) and respiratory outpatients (no acute disease, n = 15).

Results

Our full standard operating procedure for the assay is provided. Patients with severe CAP had significantly decreased neutrophil function compared to moderate severity disease (median phagocytic index 2.8 vs. 18.0, p = 0.01

SUBMITTER: Reine J 

PROVIDER: S-EPMC7140487 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature