Assessing Gammapapillomavirus infections of mucosal epithelia with two broad-spectrum PCR protocols.

ABSTRACT:

Background

Human papillomaviruses (HPVs) have been divided into mucosal and cutaneous types according to their primary epithelial tissue tropism. However, recent studies showed the presence of several cutaneous types in mucosal lesions and healthy mucosa from different anatomical sites.

Methods

Here, the HPV prevalence and type-specific distribution were assessed in a variety of mucosal samples from 435 individuals using a combination of two established broad-spectrum primer systems: Gamma-PV PCR and CUT PCR.

Results

Overall HPV prevalence in anal canal swabs, cervical cancer biopsies, genital warts and oral swabs was 85, 47, 62 and 4%, respectively. In anal canal swabs, Alpha-PVs were most frequently found (59%), followed by Gamma- (37%) and Beta-PVs (4%). The preval

SUBMITTER: Bolatti EM 

PROVIDER: S-EPMC7140492 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature