Basal-Type Breast Cancer Stem Cells Over-Express Chromosomal Passenger Complex Proteins.

ABSTRACT: (1) Aim: In the present paper we analyzed the transcriptome of CSCs (Cancer Stem Cells), in order to find defining molecular processes of breast cancer. (2) Methods: We performed RNA-Seq from CSCs isolated from the basal cell line MDA-MB-468. Enriched processes and networks were studied using the IPA (Ingenuity Pathway Analysis) tool. Validation was performed with qRT-PCR and the analysis of relevant genes was evaluated by overexpression, flow cytometry and in vivo zebrafish studies. Finally, the clinical relevance of these results was assessed using reported cohorts. (3) Results: We found that CSCs presented marked differences from the non-CSCs, including enrichment in transduction cascades related to stemness, cellular growth, proliferation and apoptosis. Interestingly, CSCs overexpresse

SUBMITTER: Schwarz-Cruz Y Celis A 

PROVIDER: S-EPMC7140627 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature