Accurate detection of short and long active ORFs using Ribo-seq data.

ABSTRACT:

Motivation

Ribo-seq, a technique for deep-sequencing ribosome-protected mRNA fragments, has enabled transcriptome-wide monitoring of translation in vivo. It has opened avenues for re-evaluating the coding potential of open reading frames (ORFs), including many short ORFs that were previously presumed to be non-translating. However, the detection of translating ORFs, specifically short ORFs, from Ribo-seq data, remains challenging due to its high heterogeneity and noise.

Results

We present ribotricer, a method for detecting actively translating ORFs by directly leveraging the three-nucleotide periodicity of Ribo-seq data. Ribotricer demonstrates higher accuracy and robustness compared with other methods at detecting actively translating ORFs including short ORFs on multiple p

SUBMITTER: Choudhary S 

PROVIDER: S-EPMC7141849 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature