CRISPRitz: rapid, high-throughput and variant-aware in silico off-target site identification for CRISPR genome editing.

ABSTRACT:

Motivation

Clustered regularly interspaced short palindromic repeats (CRISPR) technologies allow for facile genomic modification in a site-specific manner. A key step in this process is the in silico design of single guide RNAs to efficiently and specifically target a site of interest. To this end, it is necessary to enumerate all potential off-target sites within a given genome that could be inadvertently altered by nuclease-mediated cleavage. Currently available software for this task is limited by computational efficiency, variant support or annotation, and assessment of the functional impact of potential off-target effects.

Results

To overcome these limitations, we have developed CRISPRitz, a suite of software tools to support the design and analysis of CRISPR/CRISPR-ass

SUBMITTER: Cancellieri S 

PROVIDER: S-EPMC7141852 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature