The DNMT1/miR-34a/FOXM1 Axis Contributes to Stemness of Liver Cancer Cells.

ABSTRACT:

Background

Whether DNA methyltransferase 1 (DNMT1)/miR-34a/FoxM1 signaling promotes the stemness of liver cancer stem cells (LCSCs) remains unclear. This study aimed to assess whether methylation-based silencing of miR-34a by DNMT1 contributes to stemness features via FoxM1 upregulation in LCSCs.

Methods

The CD133⁺ subgroup of MHCC97H cells sorted by MACS was used as LCSCs. DNMT1, BMI1, SOX2, and OCT4 mRNA levels, and miR-34a amounts were determined by qRT-PCR. DNMT1, CD44, and FoxM1 proteins were analyzed by immunoblot. Sphere and colony formation abilities were detected by respective assays. CD133⁺ cell percentages were assessed by flow cytometry. In vivo oncogenicity was evaluated using a tumor xenograft model in mi

SUBMITTER: Cao X 

PROVIDER: S-EPMC7142390 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature