Developing Single Nucleotide Polymorphisms for Identification of Cod Products by RAD-Seq.

ABSTRACT: The increase in the rate of seafood fraud, particularly in the expensive fishes, forces us to verify the identity of marine products. Meanwhile, the definition of cod lacks consistency at the international level, as few standards and effective application methods are capable of accurately detecting cod species. Genetic fingerprinting is important for both certifying authenticity and traceability of fish species. In this study, we developed a method that combines DNA barcoding and the restriction-site associated DNA sequencing (RAD-Seq) approach for the identification of cod products. We first obtained 6941 high-quality single nucleotide polymorphism (SNP)s from 65.6 gigabases (Gb) of RAD-Seq raw data, and two sequences that contain SNPs were finally used to successfully identify three diff

SUBMITTER: Jiang S 

PROVIDER: S-EPMC7142540 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature