Digital Microfluidics for Single Bacteria Capture and Selective Retrieval Using Optical Tweezers.

ABSTRACT: When screening microbial populations or consortia for interesting cells, their selective retrieval for further study can be of great interest. To this end, traditional fluorescence activated cell sorting (FACS) and optical tweezers (OT) enabled methods have typically been used. However, the former, although allowing cell sorting, fails to track dynamic cell behavior, while the latter has been limited to complex channel-based microfluidic platforms. In this study, digital microfluidics (DMF) was integrated with OT for selective trapping, relocation, and further proliferation of single bacterial cells, while offering continuous imaging of cells to evaluate dynamic cell behavior. To enable this, magnetic beads coated with Salmonella Typhimurium-targeting antibodies were seeded in the microwel

SUBMITTER: Tewari Kumar P 

PROVIDER: S-EPMC7142809 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature