Standardization of Plant Microbiome Studies: Which Proportion of the Microbiota is Really Harvested?

ABSTRACT: Studies in plant-microbiome currently use diverse protocols, making their comparison difficult and biased. Research in human microbiome have faced similar challenges, but the scientific community proposed various recommendations which could also be applied to phytobiome studies. Here, we addressed the isolation of plant microbiota through apple carposphere and lettuce root microbiome. We demonstrated that the fraction of the culturable epiphytic microbiota harvested by a single wash might only represent one-third of the residing microbiota harvested after four successive washes. In addition, we observed important variability between the efficiency of washing protocols (up to 1.6-fold difference for apple and 1.9 for lettuce). QIIME2 analysis of 16S rRNA gene, showed a significant differenc

SUBMITTER: Sare AR 

PROVIDER: S-EPMC7142977 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature