Two Highly Similar Chitinases from Marine Vibrio Species have Different Enzymatic Properties.

ABSTRACT: Chitinase, as one of the most important extracellular enzymes in the marine environment, has great ecological and applied values. In this study, two chitinases (Chi1557 and Chi4668) with 97.33% amino acid sequences identity were individually found in Vibrio rotiferianus and Vibrio harveyi. They both were encoding by 561 amino acids, but differed in 15 amino acids and showed different enzymatic properties. The optimal temperature and pH ranges were 45-50 °C and pH 5.0-7.0 for Chi1557, while ~50 °C and pH 3.0-6.0 for Chi4668. K⁺, Mg²⁺, and EDTA increased the enzymatic activity of Chi4668 significantly, yet these factors were inhibitory to Chi1557. Moreover, Chi1557 degraded colloidal chitin to produce (GlcNAc)₂ and minor GlcNAc, whereas Chi4668

SUBMITTER: He X 

PROVIDER: S-EPMC7143101 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature