Isoprene Oxidation by the Gram-Negative Model bacterium Variovorax sp. WS11.

ABSTRACT: Plant-produced isoprene (2-methyl-1,3-butadiene) represents a significant portion of global volatile organic compound production, equaled only by methane. A metabolic pathway for the degradation of isoprene was first described for the Gram-positive bacterium Rhodococcus sp. AD45, and an alternative model organism has yet to be characterised. Here, we report the characterisation of a novel Gram-negative isoprene-degrading bacterium, Variovorax sp. WS11. Isoprene metabolism in this bacterium involves a plasmid-encoded iso metabolic gene cluster which differs from that found in Rhodococcus sp. AD45 in terms of organisation and regulation. Expression of iso metabolic genes is significantly upregulated by both isoprene and epoxyisoprene. T

SUBMITTER: Dawson RA 

PROVIDER: S-EPMC7143210 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature