Quinolinecarboxamides Inhibit the Replication of the Bovine Viral Diarrhea Virus by Targeting a Hot Spot for the Inhibition of Pestivirus Replication in the RNA-Dependent RNA Polymerase.

ABSTRACT: The bovine viral diarrhea virus (BVDV), a pestivirus from the family of Flaviviridae is ubiquitous and causes a range of clinical manifestations in livestock, mainly cattle. Two quinolinecarboxamide analogues were identified in a CPE-based screening effort, as selective inhibitors of the in vitro bovine viral diarrhea virus (BVDV) replication, i.e., TO505-6180/CSFCI (average EC₅₀ = 0.07 µM, SD = 0.02 µM, CC₅₀ > 100 µM) and TO502-2403/CSFCII (average EC₅₀ = 0.2 µM, SD = 0.06 µM, CC₅₀ > 100 µM). The initial antiviral activity observed for both hits against BVDV was corroborated by measuring the inhibitory effect on viral RNA synthesis and the production of infectious virus. Modification of the substituents on the quinolinecarboxamide

SUBMITTER: Musiu S 

PROVIDER: S-EPMC7144022 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature