Marked Neurospora crassa Strains for Competition Experiments and Bayesian Methods for Fitness Estimates.

ABSTRACT: The filamentous fungus Neurospora crassa, a model microbial eukaryote, has a life cycle with many features that make it suitable for studying experimental evolution. However, it has lacked a general tool for estimating relative fitness of different strains in competition experiments. To remedy this need, we constructed N. crassa strains that contain a modified csr-1 locus and developed an assay for detecting the proportion of the marked strain using a post PCR high resolution melting assay. DNA extraction from spore samples can be performed on 96-well plates, followed by a PCR step, which allows many samples to be processed with ease. Furthermore, we suggest a Bayesian approach for estimating relative fitness from competition experiments that takes into account the unc

SUBMITTER: Kronholm I 

PROVIDER: S-EPMC7144071 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature