BlobToolKit - Interactive Quality Assessment of Genome Assemblies.

ABSTRACT: Reconstruction of target genomes from sequence data produced by instruments that are agnostic as to the species-of-origin may be confounded by contaminant DNA. Whether introduced during sample processing or through co-extraction alongside the target DNA, if insufficient care is taken during the assembly process, the final assembled genome may be a mixture of data from several species. Such assemblies can confound sequence-based biological inference and, when deposited in public databases, may be included in downstream analyses by users unaware of underlying problems. We present BlobToolKit, a software suite to aid researchers in identifying and isolating non-target data in draft and publicly available genome assemblies. BlobToolKit can be used to process assembly, read and analysis files f

SUBMITTER: Challis R 

PROVIDER: S-EPMC7144090 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature