Hydrophobic Adsorption Followed by Desorption with Ethanol-Water for Recovery of Penicillin G from Fermentation Broth.

ABSTRACT: The hydrophobic adsorption is an alternative to traditional organic solvent extraction for the recovery and purification of Penicillin G (PenG). However, there is a lack of information concerning the effect of process variables and technical feasibility while balancing product degradation. After assessing the integrity of PenG under different conditions, Amberlite XAD-4 was selected from among three different adsorbents. During the batch process using only 0.05 gXAD-4/mLmedium, the adsorption yield increased from 36% at pH 6 to 44% at pH 4. More than 90% of the antibiotic was captured from the fermentation broth using 0.083 gXAD-4/mLmedium in a 45 min batch performed at pH 4 and 4 °C. Moreover, there was no PenG degradation. The desorption conditions were evaluated, and 95% of the antibiot

SUBMITTER: de Barros ANC 

PROVIDER: S-EPMC7144136 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature