Unravelling the proteomic landscape of extracellular vesicles in prostate cancer by density-based fractionation of urine.

ABSTRACT: Extracellular vesicles (EV) are increasingly being recognized as important vehicles of intercellular communication and promising diagnostic and prognostic biomarkers in cancer. Despite this enormous clinical potential, the plethora of methods to separate EV from biofluids, providing material of highly variable purity, and lacking knowledge regarding methodological repeatability pose a barrier to clinical translation. Urine is considered an ideal proximal fluid for the study of EV in urological cancers due to its direct contact with the urogenital system. We demonstrate that density-based fractionation of urine by bottom-up Optiprep density gradient centrifugation separates EV and soluble proteins with high specificity and repeatability. Mass spectrometry-based proteomic analysis of urinary

SUBMITTER: Dhondt B 

PROVIDER: S-EPMC7144211 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature