In Vivo Imaging of the Coupling between Neuronal and CREB Activity in the Mouse Brain.

ABSTRACT: Sensory experiences cause long-term modifications of neuronal circuits by modulating activity-dependent transcription programs that are vital for regulation of long-term synaptic plasticity and memory. However, it has not been possible to precisely determine the interaction between neuronal activity patterns and transcription factor activity. Here we present a technique using two-photon fluorescence lifetime imaging (2pFLIM) with new FRET biosensors to chronically image in vivo signaling of CREB, an activity-dependent transcription factor important for synaptic plasticity, at single-cell resolution. Simultaneous imaging of the red-shifted CREB sensor and GCaMP permitted exploration of how experience shapes the interplay between CREB and neuronal activity in the neocortex of awake mice. Dar

SUBMITTER: Laviv T 

PROVIDER: S-EPMC7144870 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature