FIN-Seq: transcriptional profiling of specific cell types from frozen archived tissue of the human central nervous system.

ABSTRACT: Thousands of frozen, archived tissue samples from the human central nervous system (CNS) are currently available in brain banks. As recent developments in RNA sequencing technologies are beginning to elucidate the cellular diversity present within the human CNS, it is becoming clear that an understanding of this diversity would greatly benefit from deeper transcriptional analyses. Single cell and single nucleus RNA profiling provide one avenue to decipher this heterogeneity. An alternative, complementary approach is to profile isolated, pre-defined cell types and use methods that can be applied to many archived human tissue samples that have been stored long-term. Here, we developed FIN-Seq (Frozen Immunolabeled Nuclei Sequencing), a method that accomplishes these goals. FIN-Seq uses immun

SUBMITTER: Amamoto R 

PROVIDER: S-EPMC7145626 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature