Presenting a codon-optimized palette of fluorescent proteins for use in Candida albicans.

ABSTRACT: Fluorescent proteins with varying colors are indispensable tools for the life sciences research community. These fluorophores are often developed for use in mammalian systems, with incremental enhancements or new versions published frequently. However, the successful application of these labels in other organisms in the tree of life, such as the fungus Candida albicans, can be difficult to achieve due to the difficulty in engineering constructs for good expression in these organisms. In this contribution, we present a palette of Candida-optimized fluorescent proteins ranging from cyan to red and assess their application potential. We also compare a range of reported expression optimization techniques, and find that none of these strategies is generally applicable, and that even very closel

SUBMITTER: Van Genechten W 

PROVIDER: S-EPMC7145796 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature