Photocrosslinking of cDNA Display Molecules with Their Target Proteins as a New Strategy for Peptide Selection.

ABSTRACT: Binding peptides for given target molecules are often selected in vitro during drug discovery and chemical biology research. Among several display technologies for this purpose, complementary DNA (cDNA) display (a covalent complex of a peptide and its encoding cDNA linked via a specially designed puromycin-conjugated DNA) is unique in terms of library size, chemical stability, and flexibility of modification. However, selection of cDNA display libraries often suffers from false positives derived from non-specific binding. Although rigorous washing is a straightforward solution, this also leads to the loss of specific binders with moderate affinity because the interaction is non-covalent. To address this issue, herein, we propose a method to covalently link cDNA display molecules with their

SUBMITTER: Terai T 

PROVIDER: S-EPMC7146492 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature