Breast cancer bone metastases are attenuated in a Tgif1-deficient bone microenvironment.

ABSTRACT:

Background

Osteoclast activation is a hallmark of breast cancer-induced bone disease while little is known about the role of osteoblasts in this process. Recently, we identified the homeodomain protein TG-interacting factor-1 (Tgif1) as a crucial regulator of osteoblast function. In this study, we demonstrate that lack of Tgif1 also restricts the progression of breast cancer bone metastases.

Methods

Transwell migration assays were used to investigate the osteoblast-breast cancer cell interaction in vitro. Molecular analyses included RNA sequencing, immunoblotting, and qRT-PCR. To determine the role of Tgif1 in metastatic bone disease, 4T1 breast cancer cells were injected intracardially into mice with a germ line deletion of Tgif1 (Tgif1^-/-) or control littermates

SUBMITTER: Haider MT 

PROVIDER: S-EPMC7146874 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature