Molecular interplay between linc01134 and YY1 dictates hepatocellular carcinoma progression.

ABSTRACT:

Background

Revealing the mechanical role of long non-coding RNAs (lncRNAs) in tumorigenesis can contribute to novel therapeutic target for cancers. The regulatory role of linc01134 in hepatocellular carcinoma (HCC) has not been studied yet.

Materials and methods

qRT-PCR and western blot were conducted to measure relevant RNA and protein expressions. CCK-8, colony formation, EdU, flow cytometry, wound-healing, transwell assays and xenograft experiments were performed to determine the role of linc01134 in HCC. ChIP and luciferase reporter assays were performed to analyze the effects of Yin Yang-1 (YY1) on linc01134 transcription activity. Relevant mechanical experiments were performed to verify interaction between relative genes.

Results

YY1 enhanced linc01134 transcrip

SUBMITTER: Rong Z 

PROVIDER: S-EPMC7146959 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature