Establishment of cell lines with porcine spermatogonial stem cell properties.

ABSTRACT:

Background

Spermatogonial stem cells (SSCs) are capable of both self-renewal and differentiation to mature functional spermatozoa, being the only adult stem cells in the males that can transmit genetic information to the next generation. Porcine SSCs hold great value in transgenic pig production and in establishment of porcine models for regenerative medicine. However, studies and applications of porcine SSCs have been greatly hampered by the low number of SSCs in the testis as well as the lack of an ideal stable long-term culture system to propagate porcine SSCs perpetually.

Results

In the present study, by lentiviral transduction of plasmids expressing the simian virus 40 (SV40) large T antigen into porcine primary SSCs, we developed two immortalized cell lines with porcin

SUBMITTER: Zheng Y 

PROVIDER: S-EPMC7146966 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature