Comparison of culture-dependent and culture-independent molecular methods for characterization of vaginal microflora.

ABSTRACT:

Purpose

To date, molecular methods that circumvent the limitations of traditional culture methods have not been used to describe the vaginal microflora in India. Here, we compared culture and culture-independent molecular methods in characterizing the vaginal microbiota in Indian women.

Methodology

Culture methods involved traditional cultivation on Rogosa and sheep blood agar, whereas culture-independent methods bypassed a culturing step by performing broadrange 16S rDNA PCR on DNA isolated directly from vaginal swabs.

Results

A total of 13 women were included in the study, of which five were characterized as healthy, two were bacterial vaginosis intermediate and six were bacterial vaginosis positive according to Nugent scoring. Lactobacillus jensenii was detected mo

SUBMITTER: Pandya S 

PROVIDER: S-EPMC7147076 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature