Reprogramming of sugar transport pathways in Escherichia coli using a permeabilized SecY protein-translocation channel.

ABSTRACT: In the initial step of sugar metabolism, sugar-specific transporters play a decisive role in the passage of sugars through plasma membranes into cytoplasm. The SecY complex (SecYEG) in bacteria forms a membrane channel responsible for protein translocation. The present work shows that permeabilized SecY channels can be used as nonspecific sugar transporters in Escherichia coli. SecY with the plug domain deleted allowed the passage of glucose, fructose, mannose, xylose, and arabinose, and, with additional pore-ring mutations, facilitated lactose transport, indicating that sugar passage via permeabilized SecY was independent of sugar stereospecificity. The engineered E. coli showed rapid growth on a wide spectrum of monosaccharides and benefited from the elimination of transport saturation,

SUBMITTER: Guo Q 

PROVIDER: S-EPMC7147117 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature