Neutrophil L-Plastin Controls Ocular Paucibacteriality and Susceptibility to Keratitis.

ABSTRACT: Why ocular mucosa is paucibacterial is unknown. Many different mechanisms have been suggested but the comprehensive experimental studies are sparse. We found that a deficiency in L-plastin (LCP1), an actin bundling protein, resulted in an ocular commensal overgrowth, characterized with increased presence of conjunctival Streptococcal spp. The commensal overgrowth correlated with susceptibility to P. aeruginosa-induced keratitis. L-plastin knock-out (KO) mice displayed elevated bacterial burden in the P. aeruginosa-infected corneas, altered inflammatory responses, and compromised bactericidal activity. Mice with ablation of LPL under the LysM Cre (LysM. Cre ^pos LPL ^fl/fl ) and S100A8 Cre (S100A8.Cre

SUBMITTER: Lu X 

PROVIDER: S-EPMC7147296 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature