Human adipose-derived mesenchymal stem cells accelerate decellularized neobladder regeneration.

ABSTRACT: Decellularized natural bladder matrices (neobladders) represent an exciting means to regenerate the bladder following bladder cancer-associated cystectomy. In this study, we compare the evolution of decellularized matrices with recellularized matrices by seeding it with human adipose-derived mesenchymal stem cells (ADSC) after implantation following partial cystectomy in rats. We discovered significant anatomical differences since 10 days after neobladder implantation with the ADSC-containing matrices promoting a significant recovery of mature p63- and cytokeratin 7-positive urothelium. We also discovered significantly induced expression of the vimentin mesoderm marker in the submucosal layer in ADSC-seeded matrices. Interestingly, we found a higher expression of smooth muscle actin in tra

SUBMITTER: Moreno-Manzano V 

PROVIDER: S-EPMC7147364 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature