Molecular Events Controlling Cessation of Trunk Neural Crest Migration and Onset of Differentiation.

ABSTRACT: Neural crest cells (NCC) migrate extensively in vertebrate embryos to populate diverse derivatives including ganglia of the peripheral nervous system. Little is known about the molecular mechanisms that lead migrating trunk NCC to settle at selected sites in the embryo, ceasing their migration and initiating differentiation programs. To identify candidate genes involved in these processes, we profiled genes up-regulated in purified post-migratory compared with migratory NCC using a staged, macroarrayed cDNA library. A secondary screen of in situ hybridization revealed that many genes are specifically enhanced in neural crest-derived ganglia, including macrophage migration inhibitory factor (MIF), a ligand for CXCR4 receptor. Through in vivo and in vitro assays, we foun

SUBMITTER: Lee VM 

PROVIDER: S-EPMC7147452 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature