PELP1 promotes glioblastoma progression by enhancing Wnt/β-catenin signaling.

ABSTRACT:

Background

Glioblastoma (GBM) is a deadly neoplasm of the central nervous system. The molecular mechanisms and players that contribute to GBM development is incompletely understood.

Methods

The expression of PELP1 in different grades of glioma and normal brain tissues was analyzed using immunohistochemistry on a tumor tissue array. PELP1 expression in established and primary GBM cell lines was analyzed by Western blotting. The effect of PELP1 knockdown was studied using cell proliferation, colony formation, migration, and invasion assays. Mechanistic studies were conducted using RNA-seq, RT-qPCR, immunoprecipitation, reporter gene assays, and signaling analysis. Mouse orthotopic models were used for preclinical evaluation of PELP1 knock down.

Results

Nuclear receptor

SUBMITTER: Sareddy GR 

PROVIDER: S-EPMC7147719 | biostudies-literature | 2019 May-Dec

REPOSITORIES: biostudies-literature