The dynamics of actin network turnover is self-organized by a growth-depletion feedback.

ABSTRACT: The dynamics of actin networks is modulated by a machinery consisting of actin binding proteins that control the turnover of filaments in space and time. To study this complex orchestration, in vitro reconstitution approaches strive to project actin dynamics in ideal, minimal systems. To this extent we reconstitute a self-supplying, dense network of globally treadmilling filaments. In this system we analyze growth and intrinsic turnover by means of FRAP measurements and thereby demonstrate how the depletion of monomers and actin binding partners modulate the dynamics in active actin networks. The described effects occur only in dense networks, as single filament dynamics are unable to produce depletion effects to this extent. Furthermore, we demonstrate a synergistic relationship between t

SUBMITTER: Bleicher P 

PROVIDER: S-EPMC7148320 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature