Coordination of mRNA and tRNA methylations by TRMT10A.

ABSTRACT: The posttranscriptional modification of messenger RNA (mRNA) and transfer RNA (tRNA) provides an additional layer of regulatory complexity during gene expression. Here, we show that a tRNA methyltransferase, TRMT10A, interacts with an mRNA demethylase FTO (ALKBH9), both in vitro and inside cells. TRMT10A installs N¹-methylguanosine (m¹G) in tRNA, and FTO performs demethylation on N⁶-methyladenosine (m⁶A) and N⁶,2'-O-dimethyladenosine (m⁶A_m) in mRNA. We show that TRMT10A ablation not only leads to decreased m¹G in tRNA but also significantly increases m⁶A levels in mRNA. Cross-linking and immunoprecipitation, followed by high-throughput sequencing results show that

SUBMITTER: Ontiveros RJ 

PROVIDER: S-EPMC7149399 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature