In vitro cell culture of patient derived malignant pleural and peritoneal effusions for personalised drug screening.

ABSTRACT:

Background

Malignant serous effusion (MSE) denotes a manifestation of metastatic disease with typical high concentrations of both cancer and immune cells, making them an ideal resource for in vitro cytologic studies. Hence, the aim of the study was to investigate the features of 2D and 3D MSE culture systems as well as their feasibilities for in vitro drug screening.

Methods

Pleural and peritoneal effusions from 8 patients were collected and processed for 2D monolayer and 3D hanging drop cell culture into GravityPLUS™ plates. Representative markers for cell components, proliferation rate and tumour classification were investigated by immunohistochemistry, followed by absolute quantification using a digitalised image analysis approach. Further, we implemented another 3D cell

SUBMITTER: Wu CG 

PROVIDER: S-EPMC7149866 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature