Aggravated brain injury after neonatal hypoxic ischemia in microglia-depleted mice.

ABSTRACT:

Background

Neuroinflammation plays an important role in neonatal hypoxic-ischemic encephalopathy (HIE). Although microglia are largely responsible for injury-induced inflammatory response, they play beneficial roles in both normal and disease states. However, the effects of microglial depletion on neonatal HIE remain unclear.

Methods

Tamoxifen was administered to Cx3cr1^CreER/+Rosa26^DTA/+ (microglia-depleted model) and Cx3cr1^CreER/+Rosa26^DTA/- (control) mice at P8 and P9 to assess the effect of microglial depletion. The density of microglia was quantified using Iba-1 staining. Moreover, the proportion of resident microglia after the HI insult was analyzed using flow cytometric analysis. At P10, the HI insult was conducted using the

SUBMITTER: Tsuji S 

PROVIDER: S-EPMC7149909 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature