The WW domains dictate isoform-specific regulation of YAP1 stability and pancreatic cancer cell malignancy.

ABSTRACT: YAP1 is a key mediator of the Hippo pathway capable of exerting a profound effect on organ size as well as tumorigenesis. Alternative mRNA splicing of human YAP1 results in at least 8 protein isoforms that differ within the 2^nd WW motif and the transcriptional activation domain. Methods: To investigate the isoform-specific differences in their mRNA expression, transcriptional activity and tumor-promoting function, we cloned cDNA encoding all of the eight YAP1 protein isoforms. Then, we examined their mRNA expression, subcellular localization, transcriptional regulation properties, interactions with key regulatory partners, and protein stability in response to changes in cell density, as well as their effects on pancreatic cancer cell malignancy both in vitro and

SUBMITTER: Guo Q 

PROVIDER: S-EPMC7150473 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature