Recombinant expression of nanobodies and nanobody-derived immunoreagents.

ABSTRACT: Antibody fragments for which the sequence is available are suitable for straightforward engineering and expression in both eukaryotic and prokaryotic systems. When produced as fusions with convenient tags, they become reagents which pair their selective binding capacity to an orthogonal function. Several kinds of immunoreagents composed by nanobodies and either large proteins or short sequences have been designed for providing inexpensive ready-to-use biological tools. The possibility to choose among alternative expression strategies is critical because the fusion moieties might require specific conditions for correct folding or post-translational modifications. In the case of nanobody production, the trend is towards simpler but reliable (bacterial) methods that can substitute for more cu

SUBMITTER: de Marco A 

PROVIDER: S-EPMC7151424 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature