High-content imaging of 3D-cultured neural stem cells on a 384-pillar plate for the assessment of cytotoxicity.

ABSTRACT: The assessment of neurotoxicity has been performed traditionally with animals. However, in vivo studies are highly expensive and time-consuming, and often do not correlate to human outcomes. Thus, there is a need for cost-effective, high-throughput, highly predictive alternative in vitro test methods based on early markers of mechanisms of toxicity. High-content imaging (HCI) assays performed on three-dimensionally (3D) cultured cells could provide better understanding of the mechanism of toxicity needed to predict neurotoxicity in humans. However, current 3D cell culture systems lack the throughput required for screening neurotoxicity against a large number of chemicals. Therefore, we have developed miniature 3D neural stem cell (NSC) culture on a unique 384-pillar plate, which is complem

SUBMITTER: Joshi P 

PROVIDER: S-EPMC7152566 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature