Flux Balance Analysis for Media Optimization and Genetic Targets to Improve Heterologous Siderophore Production.

ABSTRACT: Siderophores are small molecule metal chelators secreted in sparse quantities by their native microbial hosts but can be engineered for enhanced production from heterologous hosts like Escherichia coli. These molecules have been proved to be capable of binding heavy metals of commercial and/or environmental interest. In this work, we incorporated, as needed, the appropriate pathways required to produce several siderophores (anguibactin, vibriobactin, bacillibactin, pyoverdine, and enterobactin) into the base E. coli K-12 MG1655 metabolic network model to computationally predict, via flux balance analysis methodologies, gene knockout targets, gene over-expression targets, and media modifications capable of improving siderophore reaction flux. E. coli metabolism proved supportive for the und

SUBMITTER: Swayambhu G 

PROVIDER: S-EPMC7152677 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature