Peripapillary Intravitreal Injection Improves AAV-Mediated Retinal Transduction.

ABSTRACT: The intravitreal (IVT) injection method is a choice when targeting the inner retina for gene therapy. However, the transduction efficiency of adeno-associated virus (AAV) vectors administered by the IVT route is usually low and may be affected by several factors. To improve the transduction efficiency, we developed a novel illuminated long-needle attached injection system and injected AAV2-CMV (cytomegalovirus)-EGFP in front of the retina in rabbit eyes. Ophthalmological examinations were performed and the levels of pro-inflammatory cytokines in the aqueous humor were assessed at the baseline and 1 month, and the results were compared with those of the conventional injection method. Retinal tissues were used for immunohistochemistry. In the ophthalmological examinations, no significant inf

SUBMITTER: Madrakhimov SB 

PROVIDER: S-EPMC7152690 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature