Biochemical characterization of acyl activating enzymes for side chain moieties of Taxol and its analogs.

ABSTRACT: Taxol (paclitaxel) is a very widely used anticancer drug, but its commercial sources mainly consist of stripped bark or suspension cultures of members of the plant genus Taxus. Taxol accumulates as part of a complex mixture of chemical analogs, termed taxoids, which complicates its production in pure form, highlighting the need for metabolic engineering approaches for high-level Taxol production in cell cultures or microbial hosts. Here, we report on the characterization of acyl-activating enzymes (AAEs) that catalyze the formation of CoA esters of different organic acids relevant for the N-substitution of the 3-phenylisoserine side chain of taxoids. On the basis of similarities to AAE genes of known function from other organisms, we identified candidate genes in publicly available

SUBMITTER: Srividya N 

PROVIDER: S-EPMC7152758 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature