The Class III PI3K/Beclin-1 Autophagic Pathway Participates in the mmLDL-Induced Upregulation of ET_A Receptor in Mouse Mesenteric Arteries.

ABSTRACT: Minimally modified low-density lipoprotein (mmLDL) is a risk factor for cardiovascular diseases. The current study explored the effect of mmLDL on the endothelin type A (ETA) receptor in mouse mesenteric arteries in vivo, as well as the role of autophagy in this process. mmLDL was injected via the caudal vein, and the Class III PI3K autophagic pathway inhibitor 3-methyladenine (3-MA) was injected intraperitoneally. The animals were divided into physiological saline (NS), mmLDL, and mmLDL + 3-MA groups. The dose-effect curve of endothelin-1- (ET-1-) induced mesenteric artery contraction was measured using myography, while ET_A receptor mRNA expression was detected using real-time polymerase chain reactions, and the protein levels of the ET_A receptor, class III PI

SUBMITTER: Xie X 

PROVIDER: S-EPMC7152935 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature