Multicentre Harmonisation of a Six-Colour Flow Cytometry Panel for Naive/Memory T Cell Immunomonitoring.

ABSTRACT:

Background

Personalised medicine in oncology needs standardised immunological assays. Flow cytometry (FCM) methods represent an essential tool for immunomonitoring, and their harmonisation is crucial to obtain comparable data in multicentre clinical trials. The objective of this study was to design a harmonisation workflow able to address the most effective issues contributing to intra- and interoperator variabilities in a multicentre project.

Methods

The Italian National Institute of Health (Istituto Superiore di Sanità, ISS) managed a multiparametric flow cytometric panel harmonisation among thirteen operators belonging to five clinical and research centres of Lazio region (Italy). The panel was based on a backbone mixture of dried antibodies (anti-CD3, anti-CD4, anti-CD8,

SUBMITTER: Macchia I 

PROVIDER: S-EPMC7153001 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature