Essential roles of HDAC1 and 2 in lineage development and genome-wide DNA methylation during mouse preimplantation development.

ABSTRACT: Epigenetic modifications, including DNA methylation and histone modifications, are reprogrammed considerably following fertilization during mammalian early embryonic development. Incomplete epigenetic reprogramming is a major factor leading to poor developmental outcome in embryos generated by assisted reproductive technologies, such as somatic cell nuclear transfer. However, the role of histone modifications in preimplantation development is poorly understood. Here, we show that co-knockdown (cKD) of Hdac1 and 2 (but not individually) resulted in developmental failure during the morula to blastocyst transition. This outcome was also confirmed with the use of small-molecule HDAC1/2-specific inhibitor FK228. We observed reduced cell proliferation and increased incidence of apo

SUBMITTER: Zhao P 

PROVIDER: S-EPMC7153544 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature