Functional characterization of maize heat shock transcription factor gene ZmHsf01 in thermotolerance.

ABSTRACT:

Background

Heat waves can critically influence maize crop yields. Plant heat shock transcription factors (HSFs) play a key regulating role in the heat shock (HS) signal transduction pathway.

Method

In this study, a homologous cloning method was used to clone HSF gene ZmHsf01 (accession number: MK888854) from young maize leaves. The transcript levels of ZmHsf01 were detected using qRT-PCR in different tissues and treated by HS, abscisic acid (ABA), hydrogen peroxide (H₂O₂), respectively, and the functions of gene ZmHsf01 were studied in transgenic yeast and Arabidopsis.

Result

ZmHsf01 had a coding sequence (CDS) of 1176 bp and encoded a protein consisting of 391 amino acids. The homologous analysis results showed

SUBMITTER: Zhang H 

PROVIDER: S-EPMC7153558 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature